I did it for five weeks, and noticed big improved with my knee and calf injury. I also used 80mg (50MG GHKU-Cu, and 10mg others), reconstituted with 3ml BAC water. I did twice a day, 12units each. (works out to be 2mg/400mcg/400mcg/400mcg per injection. I injected both subq, and in the area where the issue is,i.e. knee or calf muscle. I don't think it makes difference between localized vs. target area.