Community Research MOTS-C Peptide - Overview, Dosing, and Reviews

HarleyGuy · Mar 28, 2026

MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview
<<updated 05Jun2026>>

References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance
MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome
MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway
MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis
MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet
MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA
Most evidence is preclinical (animal-based)
Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)
MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects
Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise
Fasting
Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance
Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.
MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis
Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance
Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans
"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)
"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."
"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."
"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)
"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support
Endurance and performance
Metabolic efficiency
Healthy aging
Liver and organ support
Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback
Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)
Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide
MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research
If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list
Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

EVO members' MOTS-c daily dosing ranges: 500mcg - 20mg
<<updated 05Jun2026>>

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here

Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log powered by @War Born Peptides
@Darth_Vader 5mg e3d You can follow his log here

Darth Vader's 2026 Cycle Log
@Demon_throne 4mg ED "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log powered by @Unitedau
@jwright1275 4mg e3d You can follow his log here

jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here

G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here

Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here

HarleyGuy's 2026 Cycle Log powered by @biogenix
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log here

Dreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here

RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here

FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log here

Eveflorence's 2026 Cycle Log
@Doctakay 500mcg-2mg pwo/ED You can follow his log here

Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here

jenkemj's 2026 Cycle Log powered by @Prymal
@willipede 1mg ED You can follow his log here

willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here

Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log here

TheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log here

madcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here

Flash_is_Fast's 2026 Cycle Log
@fatboy999 1mg ED You can follow his log here Fatboy999's 2026 Cycle Log
@OwEv751 2mg EOD You can follow his log here

OwEv751's 2026 Peptide Cycle Log
@gar71 1-2mg ED You can follow his log here

gar71's 2026 Cycle Log
@Thoranosaurus_flex 1mg ED You can follow his log here Thoranosaurus_flex's Cycle Log
@treebeard 3.5mg E3D You can follow his log here

treebeard's Cycle Log
@fingers86 ~~2mg ED~~ *updated to 20mg ED 07Apr* You can follow his log here fingers86's Cycle Log
@Nelsonhaha 4mg pwo You can follow his log here

Nelsonhaha's Log
@Jaxss 1-1.5mg pwo You can follow his log here Jaxss' Log
@theJimJim 2mg ED You can follow his log here

theJimJim's Log
@PrinceDaddy 1mg ED You can follow his log here

PrinceDaddy's Log
@Warthog61 1-1.5mg ED You can follow his log here

Warthog61's Log
@kcates 1mg ED You can follow his log here

kcates' Cycle Log
@8enni8lanko 1mg ED You can follow his log here

8enni8lanko's Cycle Log
@Yeahnah 2mg EOD pwo You can follow his log here

Yeahnah's Cycle Log
@Leozin 5mg EOD You can follow his log here

leolkn's Cycle Log
@Dumptruck 5mg REST DAYS You can follow his log here Dumptruck's Cycle Log
@Nickh27 2mg ED You can follow his log here

Nickh27's Log
@Katsumi 1mg ED You can follow her log here Katsumi's Log
@HotHoney 1mg ED You can follow her log here

hothoney's Log

Thanks for reading,
HarleyGuy

Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast @Yeahnah @Dumptruck @Leozin @Nickh27 @Katsumi @HotHoney
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry @Shakey @wye @HarleyGuy @BritishCanadian @zucchini @LevButlerov @OxygenPharm @abaltered @Robarock @BertaBoy @haldencapital @Farmboy
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @HarleyGuy @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @BeMe @Farmboy @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules @RedNeck @Acerico34 @Warthog61 @James Creeper @Simoneme @Robarock @CookieBaah @Struman @zucchini @Struman @CookieBaah @Robarock @Grumpy @catdadironman @wye @Asthetek23 @electus
Interested Participants:
@Panda22 @Sheshredz

rayray01 · Mar 28, 2026

Love this very informative

fatboy999 · Mar 28, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Thanks for the reminder, going to pin motsc now before I head gym hehe

Nasser1997o2 · Mar 28, 2026

sold, how much and how often?

willipede · Mar 28, 2026

Very nice write up, ive had really good experiences using mots-c at 1mg per day. I havent explored the effects of increased dosing.

But I found substantial benefits toward energy, mental clarity, focus. These are the main reason I like to utilise it in my protocols

I am yet to see any noticable side effects.

Health markers have been improving over time and cant see anything negatively impacted with the addition of mots-c

jenkemj · Mar 28, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Fantastic read! Great work @HarleyGuy !

LevButlerov · Mar 28, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Well written I find mots-c is works well with low dose cardarine as well (gw 501516)

@HarleyGuy

toddthelineman · Mar 28, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Great write up! I wish that mots c had worked for me. I could always use more energy and mental clarity. I kept increasing the dose until I ran out and gave up.

RawCutlery · Mar 28, 2026

Again I love the science behind this bro, I'll be done with my Kinesiology degree in the next 19 months. After that I don't know if I want to go into exercise physiology or get my PhD and go Physical Therapy route, but I love all of this and the very benefits behind this peptide.

toddthelineman · Mar 28, 2026

RawCutlery said:
Again I love the science behind this bro, I'll be done with my Kinesiology degree in the next 19 months. After that I don't know if I want to go into exercise physiology or get my PhD and go Physical Therapy route, but I love all of this and the very benefits behind this peptide.

I want a body builder physical therapist! My first PT wrote “well developed male” in my report

. He had fun with me.

RawCutlery · Mar 28, 2026

toddthelineman said:
I want a body builder physical therapist! My first PT wrote “well developed male” in my report . He had fun with me.

hahahahah!!! Indeed he did..

fingers86 · Mar 28, 2026

Great read, i have a full kit of mots 40mg, i might even try 5mg am fasted and 5mg night to see difference in fasted glucose

Grumpy · Mar 28, 2026

Perfect been looking for some quality info on mot c

eazy_ · Mar 28, 2026

excellent information. thank you for sharing.

Robules · Mar 28, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Super appreciated information

Flash_is_Fast · Mar 28, 2026

25mg ed - so about abou $150/d for clinial trial dosing.

Ive started this week along with 2 vo2 sessions a week, and 1 longer endurance ride. Tracking changes with a power meter (+/- 2% accuracy, and garmin hr strap (bang on accurate). 1mg pre cardio. I'll update if something above normal happens.

HarleyGuy · Mar 29, 2026

rayray01 said:
Love this very informative

Thanks @rayray01 glad you're getting into the science of it with us too!

HarleyGuy · Mar 29, 2026

fatboy999 said:
Thanks for the reminder, going to pin motsc now before I head gym hehe

LOL glad I could help anyone remember to pin!

Bro what's your dose of MOTS at? I couldn't find it in your log and I don't have you down as running it.

HarleyGuy · Mar 29, 2026

Nasser1997o2 said:
sold, how much and how often?

Glad I could help you decide to try it out if you were thinking of it. Standard is 1-2mg ED to start and it can be run indefinitely but 8 weeks would be a standard. As you might have seen, some go as high as 10mg ED

HarleyGuy · Mar 29, 2026

willipede said:
Very nice write up, ive had really good experiences using mots-c at 1mg per day. I havent explored the effects of increased dosing.

But I found substantial benefits toward energy, mental clarity, focus. These are the main reason I like to utilise it in my protocols

I am yet to see any noticable side effects.

Health markers have been improving over time and cant see anything negatively impacted with the addition of mots-c

This is awesome feedback at 1mg ED @willipede thanks for contributing! Really glad to see your health markers have improved as well. I'll be getting my bloodwork results on Monday and will be posting them.

HarleyGuy · Mar 29, 2026

jenkemj said:
Fantastic read! Great work @HarleyGuy !

Thanks bro! How are you finding the 2mg ED? For some there's nothing overly noticeable and for others there's a noted positive benefit.

HarleyGuy · Mar 29, 2026

toddthelineman said:
Great write up! I wish that mots c had worked for me. I could always use more energy and mental clarity. I kept increasing the dose until I ran out and gave up.

How high a dose did you get bro?

HarleyGuy · Mar 29, 2026

RawCutlery said:
Again I love the science behind this bro, I'll be done with my Kinesiology degree in the next 19 months. After that I don't know if I want to go into exercise physiology or get my PhD and go Physical Therapy route, but I love all of this and the very benefits behind this peptide.

I'm glad you liked it bro I love the science behind it all too. I tried to write it up as easy to read as possible so as not to scare off those that aren't as into the weeds on the bio or organic chemistry of it but I sprinkled some in there.

Congrats on almost finishing Kinesiology bro that is a big time Degree! Whatever you choose I'm thinking you'll soar, you have the passion for the subject needed.

HarleyGuy · Mar 29, 2026

fingers86 said:
Great read, i have a full kit of mots 40mg, i might even try 5mg am fasted and 5mg night to see difference in fasted glucose

This would be great @fingers86 I'd love if you posted some of your impressions at higher doses here once you're done.

HarleyGuy · Mar 29, 2026

Grumpy said:
Perfect been looking for some quality info on mot c

Awesome @Grumpy !! You'll find what you need here and the right dose for you. If/when you decide to give it a run keep us posted here.

HarleyGuy · Mar 29, 2026

eazy_ said:
excellent information. thank you for sharing.

Thank you too bro for your articles you're writing that hasn't gone unnoticed with me, you're doing some great work!

HarleyGuy · Mar 29, 2026

Robules said:
Super appreciated information

Thanks @Robules and great podcast by the way!!

HarleyGuy · Mar 29, 2026

Flash_is_Fast said:
25mg ed - so about abou $150/d for clinial trial dosing.

Ive started this week along with 2 vo2 sessions a week, and 1 longer endurance ride. Tracking changes with a power meter (+/- 2% accuracy, and garmin hr strap (bang on accurate). 1mg pre cardio. I'll update if something above normal happens.

Thanks for jumping in @Flash_is_Fast! I've added your 1mg pre-cardio dose to the original thread along with your log.

The power meter and garmin will be excellent feedback for this thread thanks for that!

HarleyGuy · Mar 29, 2026

@Stylidude I remember a conversation we were having with @Flash_is_Fast about a week ago on your log about MOTS-c, you might like this research thread.

Made_Man · Mar 29, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here Flash_is_Fast's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

What a great read. Thanks for putting in the effort to put this all togeather

kamsikazee · Mar 29, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here Flash_is_Fast's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Great informative write up on Mots-C brother! Appreciate your time and effort! Its a very promising peptide. How are you going with it?

jenkemj · Mar 29, 2026

HarleyGuy said:
Thanks bro! How are you finding the 2mg ED? For some there's nothing overly noticeable and for others there's a noted positive benefit.

Hard to say really I feel like there’s a good synergy between it and the NAD+ but that’s where I feel the majority of my energy. I’d say my dose is also a lot stronger on the NAD+.

HarleyGuy · Mar 29, 2026

Made_Man said:
What a great read. Thanks for putting in the effort to put this all togeather

Thanks bro it was a couple weeks in the making and I'm glad you thought it was great, sincerely this is what makes me feel like I'm giving back!

HarleyGuy · Mar 29, 2026

kamsikazee said:
Great informative write up on Mots-C brother! Appreciate your time and effort! Its a very promising peptide. How are you going with it?

Personally I love it so far. I take it around 10am ED and it's helped me not need to nap as much and some days not at all and I only sleep from about 1am-6am most nights.

HarleyGuy · Mar 29, 2026

jenkemj said:
Hard to say really I feel like there’s a good synergy between it and the NAD+ but that’s where I feel the majority of my energy. I’d say my dose is also a lot stronger on the NAD+.

Ya I hear you on that one I'm running 1mg MOTS with 25mg NAD+ and plan on doubling up on both soon.

fingers86 · Mar 29, 2026

HarleyGuy said:
This would be great @fingers86 I'd love if you posted some of your impressions at higher doses here once you're done.

Yeah unsure if having early in the day would affect my 930-10am igf-1lr3? Currently takes mots only at 5am fasted. But might up night dose. Unsure if should increase morning or not

fatboy999 · Mar 29, 2026

HarleyGuy said:
LOL glad I could help anyone remember to pin!

Bro what's your dose of MOTS at? I couldn't find it in your log and I don't have you down as running it.

1mg a day haha

Yeahnah · Mar 29, 2026

HarleyGuy said:
Personally I love it so far. I take it around 10am ED and it's helped me not need to nap as much and some days not at all and I only sleep from about 1am-6am most nights.

Yeah that’s me when I’m out at work now mate I think I really need to jump on it hey see if it’ll help. That’s heaps for the write up!

HarleyGuy · Mar 29, 2026

fingers86 said:
Yeah unsure if having early in the day would affect my 930-10am igf-1lr3? Currently takes mots only at 5am fasted. But might up night dose. Unsure if should increase morning or not

igf1-lr3 can cause insulin resistance so in theory MOTS-c would be a good mitigator of this; I think where you'd have to simply be smart about it is that igf1-lr3 can also cause hypoglycemia (we're talking silly doses above 100mcg) and since MOTS-c helps regulate glucose metabolism you'd just have to try it out and keep a Snickers bar nearby, for example. This now makes me want to run igf1-lr3 with MOTS just to see and post results here!

HarleyGuy · Mar 29, 2026

fatboy999 said:
1mg a day haha

Haha thanks bro! You've been added to the original post as part of the MOTS club! You should see the tag pop up.

HarleyGuy · Mar 29, 2026

Yeahnah said:
Yeah that’s me when I’m out at work now mate I think I really need to jump on it hey see if it’ll help. That’s heaps for the write up!

You're very welcome brother! If you check the quotes from members in the original post as well as other logs there's lots of feedback about avoiding that 'afternoon crash'. If you try it out just start at 1mg and post your review here.

kamsikazee · Mar 29, 2026

HarleyGuy said:
Personally I love it so far. I take it around 10am ED and it's helped me not need to nap as much and some days not at all and I only sleep from about 1am-6am most nights.

Are you taking it fasted, and before your workout?

waggat · Mar 29, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here Flash_is_Fast's 2026 Cycle Log
@fatboy999 1mg ED You can follow his log here Fatboy999's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Awesome post mate. Very informative and well presented!

fingers86 · Mar 29, 2026

HarleyGuy said:
igf1-lr3 can cause insulin resistance so in theory MOTS-c would be a good mitigator of this; I think where you'd have to simply be smart about it is that igf1-lr3 can also cause hypoglycemia (we're talking silly doses above 100mcg) and since MOTS-c helps regulate glucose metabolism you'd just have to try it out and keep a Snickers bar nearby, for example. This now makes me want to run igf1-lr3 with MOTS just to see and post results here!

Yeah im not going above that. This is just keeping me going while sessions limited. Havnt read anyone going higher than that yet. Im playing safe though.

Yeahnah · Mar 29, 2026

HarleyGuy said:
You're very welcome brother! If you check the quotes from members in the original post as well as other logs there's lots of feedback about avoiding that 'afternoon crash'. If you try it out just start at 1mg and post your review here.

Sounds like a plan bro

It’ll be AM I’ll be trialing with.

HarleyGuy · Mar 29, 2026

kamsikazee said:
Are you taking it fasted, and before your workout?

I take it right before my first meal so technically fasted yes, and my workout is after my 3rd meal at 3pm with workout around 5pm
@Dreamer raised a very good argument to take it with your highest carb meal (for him it's with carbs before bed) and then there's that other camp that take it fasted as well in the AM.

HarleyGuy · Mar 29, 2026

waggat said:
Awesome post mate. Very informative and well presented!

Thanks bro glad you got something out of it!

HarleyGuy · Mar 29, 2026

fingers86 said:
Yeah im not going above that. This is just keeping me going while sessions limited. Havnt read anyone going higher than that yet. Im playing safe though.

You are a beast I love it. We needed someone like you to run it this way because human clinical data in phase 1b trials had them taking 25mg subQ ED with amazing results including liver values AST/ALT improving by a clinically significant margin.

HarleyGuy · Mar 29, 2026

Yeahnah said:
Sounds like a plan bro

It’ll be AM I’ll be trialing with.

That's a great start let's do 1mg upon waking fasted. Nice and standard way to start. Be mindful of how you feel in the afternoon or when you know you normally have your afternoon crash; for some this is 9am and for others it's more commonly 1-2pm.

fingers86 · Mar 29, 2026

HarleyGuy said:
You are a beast I love it. We needed someone like you to run it this way because human clinical data in phase 1b trials had them taking 25mg subQ ED with amazing results including liver values AST/ALT improving by a clinically significant margin.

Well i will be getting bloods done very soon as am changing from igf to superdrol 10mg for 4 weeks. Be interesting to see liver function during that. Id go 20mg per day just coz then ill get 2 days from a vile lol. That will mean i will have enough at that dose for duration of superdrol. Be amazing if liver functions are great during something extremely hepatoxic

fingers86 · Mar 29, 2026

fingers86 said:
Well i will be getting bloods done very soon as am changing from igf to superdrol 10mg for 4 weeks. Be interesting to see liver function during that. Id go 20mg per day just coz then ill get 2 days from a vile lol. That will mean i will have enough at that dose for duration of superdrol. Be amazing if liver functions are great during something extremely hepatoxic

Well just short but will buy more unless someone wants to throw some my way lol

Mobster · Mar 29, 2026

Feedback to date has been a sense of energy and best dosed in the am.

HarleyGuy · Mar 29, 2026

fingers86 said:
Be amazing if liver functions are great during something extremely hepatoxic

Not likely but let's see. If you get liver function bloods during superdrol that would be another nice bit of data for superdrol.

HarleyGuy · Mar 29, 2026

Mobster said:
Feedback to date has been a sense of energy and best dosed in the am.

Exactly right @Mobster thanks for validating!

fingers86 · Mar 29, 2026

HarleyGuy said:
Not likely but let's see. If you get liver function bloods during superdrol that would be another nice bit of data for superdrol.

Yeah plan is bloods before i start, bloods in the last week. Then 4-6 weeks after i stop to see if back to baseline and to see how it affects me. As may use again on bulk while eq kicks in. Plus for science!

HarleyGuy · Mar 29, 2026

fingers86 said:
Yeah plan is bloods before i start, bloods in the last week. Then 4-6 weeks after i stop to see if back to baseline and to see how it affects me. As may use again on bulk while eq kicks in. Plus for science!

Sounds great I'll be following, and definitely keep us posted on the high dose MOTS-c please. If you can remember please put in here on our research thread as you are only one of two members running it at 10mg.

fingers86 · Mar 29, 2026

HarleyGuy said:
Sounds great I'll be following, and definitely keep us posted on the high dose MOTS-c please. If you can remember please put in here on our research thread as you are only one of two members running it at 10mg.

I havnt been, i was running 2mg only in am. But never new it can help liver functions or seen the study doing 25mg. Im just lucky to have 9 bottles still of mots, will need to get more before i run out.

HarleyGuy · Mar 29, 2026

fingers86 said:
I havnt been, i was running 2mg only in am. But never new it can help liver functions or seen the study doing 25mg. Im just lucky to have 9 bottles still of mots, will need to get more before i run out.

Ah yes I remember, I did post that you were at 2mg ED just thought you were planning on upping to 5mg twice a day. Either way keep us posted.

stevesmi · Mar 29, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here Flash_is_Fast's 2026 Cycle Log
@fatboy999 1mg ED You can follow his log here Fatboy999's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

nice job on this man. I actually forwarded this to a bunch of my clients who have been asking about this peptide. It looks like an amazing peptide for people who need this type of boost.

ROIDDERS · Mar 29, 2026

Nasser1997o2 said:
sold, how much and how often?

2Thick · Mar 29, 2026

Another excellent job on a peptide that is really getting popular fast. Seems like a good option to take in the morning for sustained energy throughout the day.

Ulter · Mar 29, 2026

I wish I had this stuff back when I was engineering things for the theme parks. That would have helped me a lot. I think I lost a lot of time in the process.

ceo · Mar 29, 2026

bros dayum. You are a smart dude writing out this literature. I like how you explain it easily so even dumb meatheads can understand it. think i might buy this for my wife. she is lazy always sitting around the house doing nothing

ballin2504 · Mar 29, 2026

Really great write up

HarleyGuy · Mar 29, 2026

MOTS-c powered by....

If any member would like their MOTS-c "powered by" source tagged here is the place to do it let's give credit where credit is due. You can reply here and I'll add it to this post. Let's get our approved sources names out there!

@Allupfromhere
@San Andreas powered by @War Born Peptides
@Darth_Vader
@Demon_throne powered by @Unitedau
@jwright1275
@G-Merc 80
@Shakey powered by @biogenix
@HarleyGuy MOTS-c powered by @biogenix
@Dreamer
@RogueCherry
@Freakfitness
@Eveflorence
@Doctakay
@jenkemj powered by @Prymal
@willipede
@Made_Man
@TheOne1
@madcap71
@Flash_is_Fast
@fatboy999

HarleyGuy · Mar 29, 2026

ceo said:
bros dayum. You are a smart dude writing out this literature. I like how you explain it easily so even dumb meatheads can understand it. think i might buy this for my wife. she is lazy always sitting around the house doing nothing

I tried not to get too into the weeds on the science of it and just get to the punchline but the references are some really interesting studies, there's a ton of them but those 5 were super useful and the most interesting!

HarleyGuy · Mar 29, 2026

ballin2504 said:
Really great write up

Thanks bro!

Demon_throne · Mar 29, 2026

HarleyGuy said:
MOTS-c powered by....

If any member would like their MOTS-c "powered by" source tagged here is the place to do it let's give credit where credit is due. You can reply here and I'll add it to this post. Let's get our approved sources names out there!

@Allupfromhere
@San Andreas
@Darth_Vader
@Demon_throne
@jwright1275
@G-Merc 80
@Shakey powered by @biogenix
@HarleyGuy MOTS-c powered by @biogenix
@Dreamer
@RogueCherry
@Freakfitness
@Eveflorence
@Doctakay
@jenkemj
@willipede
@Made_Man
@TheOne1
@madcap71
@Flash_is_Fast
@fatboy999

My mots c comes from my pep sponsor who is no longer on evo but is on ef (@Unitedau)

jenkemj · Mar 29, 2026

HarleyGuy said:
MOTS-c powered by....

If any member would like their MOTS-c "powered by" source tagged here is the place to do it let's give credit where credit is due. You can reply here and I'll add it to this post. Let's get our approved sources names out there!

@Allupfromhere
@San Andreas
@Darth_Vader
@Demon_throne
@jwright1275
@G-Merc 80
@Shakey powered by @biogenix
@HarleyGuy MOTS-c powered by @biogenix
@Dreamer
@RogueCherry
@Freakfitness
@Eveflorence
@Doctakay
@jenkemj
@willipede
@Made_Man
@TheOne1
@madcap71
@Flash_is_Fast
@fatboy999

Powered by @Prymal bro

San Andreas · Mar 30, 2026

My Mots c coming from @War Born Peptides

HarleyGuy · Mar 30, 2026

Demon_throne said:
My mots c comes from my pep sponsor who is no longer on evo but is on ef (@Unitedau)

Thanks @Demon_throne and shout out to our sister board EF!

HarleyGuy · Mar 30, 2026

jenkemj said:
Powered by @Prymal bro

Thanks @jenkemj will add accordingly!

HarleyGuy · Mar 30, 2026

San Andreas said:
My Mots c coming from @War Born Peptides

Aesome @San Andreas will add @War Born Peptides to your spot on the original thread!

ceo · Mar 30, 2026

HarleyGuy said:
I tried not to get too into the weeds on the science of it and just get to the punchline but the references are some really interesting studies, there's a ton of them but those 5 were super useful and the most interesting!

bros keep it up! you are a beast

HarleyGuy · Mar 30, 2026

ceo said:
bros keep it up! you are a beast

Thanks bro! It's super rewarding helping others.

ceo · Mar 30, 2026

HarleyGuy said:
Thanks bro! It's super rewarding helping others.

Bros, that's true for sure. I'm helping one of the dudes I work with. He is really out of shape; he wanted to get into better shape. He's starting to eat good; I'm proud of him.

Demon_throne · Mar 30, 2026

HarleyGuy said:
Thanks @Demon_throne and shout out to our sister board EF!

I will also be pushing up to 4mg daily at some point (I'll keep you updated for when I do, do this) just need to get myself a little extra stock so I can trial it for a while with out worrying about running out to see if there is much of a difference

Tiger Salman Khan · Mar 30, 2026

Good info....thanks for sharing......

teddy_vio · Mar 31, 2026

toddthelineman said:
Great write up! I wish that mots c had worked for me. I could always use more energy and mental clarity. I kept increasing the dose until I ran out and gave up.

It didn't work for me either, didn't notice anything at all from it. I have 9 more vials of it sitting in the freezer, maybe I'll try again someday.

toddthelineman · Mar 31, 2026

teddy_vio said:
It didn't work for me either, didn't notice anything at all from it. I have 9 more vials of it sitting in the freezer, maybe I'll try again someday.

A few others have said that as well. Any theories why it wouldn’t work for you? Is your output already very high?

G-Merc 80 · Mar 31, 2026

HarleyGuy said:
MOTS-c PEPTIDE (a.k.a.: "MOTS")
A readable layman's overview

View attachment 205576

View attachment 205575
References:
https://pubmed.ncbi.nlm.nih.gov/31293078 /
https://www.nature.com/articles/s41467-020-20790-0
https://doi.org/10.1016/j.cmet.2015.02.009
Journal of Alzheimer's Drug Discovery Foundation
Human Trial 1b of MOTS-c Analogue Results
EVO - Members' Dosing and Anecdotal Reports

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

MOTS-c is a 16 amino acid peptide that is coded by the mitochondrial genome. The mitochondria are the “power generators” in cells that convert chemical energy from food into a form that cells can use. MOTS-c is a mitochondrial-derived peptide (MDP) encoded by mitochondrial DNA and involved in regulating metabolism and energy balance. It plays a key role in helping the body adapt to metabolic stress, such as exercise and caloric restriction. MOTS-c has shown to protect against insulin resistance and obesity in older test subjects, muscle catabolism in the aging, and even supports memory function in aging subjects.

Unlike most peptides, MOTS-c can travel to the nucleus and influence gene expression related to metabolism and cellular function.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

MOTS-c is a 16-amino-acid peptide encoded in the mitochondrial genome

MOTS-c targets muscle and regulates metabolism via the folate-purine-AMPK pathway

MOTS-c mediates mitochondrial regulation of insulin and metabolic homeostasis

MOTS-c protects against age- and diet-dependent insulin resistance and obesity even in the environment of a high fat diet

MOTS-c efficacy may only be clinically significant in older human subjects where it is observed to be in decline as subjects age

Important Reality Check!

MOTS-c is not approved by regulatory agencies like Health Canada or the FDA

Most evidence is preclinical (animal-based)

Long-term safety and optimal dosing in humans are unknown (EVO members' anecdotal experiences and dosing seen below)

MOTS-c has had clinically significant results in aging animal-based and human subjects and may not be efficacious to younger subjects

Not everyone may need this and this is not a promotion for you to use MOTS-c, however clinical data in rodents (and minimal human studies) shows it is very promising!

Why MOTS-C Matters For Us?

MOTS-c activates AMP-activated protein kinase (AMPK), a central regulator of cellular energy often referred to as the body’s “metabolic master switch.”

This pathway is also activated by:

Exercise

Fasting

Caloric restriction in general

Our Potential Fitness Benefits of MOTS-C

1. Increased Fat Burning & Improved Metabolism

MOTS-c has been shown to improve metabolic flexibility and prevent fat accumulation even under high-fat diet conditions in animal models.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Demonstrated reduced weight gain and improved glucose metabolism in mice

2. Improved Endurance & Physical Performance

Study Evidence (see references):

Research shows MOTS-c can enhance physical performance and mimic some adaptations typically seen with exercise.

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Improved running capacity and muscle function in older mice

3. Better Muscle Energy Utilization

MOTS-c improves how skeletal muscle uses glucose, making workouts more efficient at the cellular level.

Study Evidence (see references):

MOTS-c enhances insulin sensitivity in skeletal muscle

4. Improved Insulin Sensitivity

One of the strongest areas of evidence: MOTS-c enhances insulin sensitivity and glucose uptake.

Study Evidence (see references):

The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance

Showed improved insulin response and reduced insulin resistance

5. Longevity & Recovery Potential

MOTS-c levels decline with age, and supplementation in animal models has been linked to improved physical function and metabolic health in older subjects.

Study Evidence (see references):

MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis

Human Research (Early but Emerging)

While most data comes from animal studies, early human research suggests MOTS-c is relevant to fitness and metabolic health. A MOTS-c analogue was shown to significantly decrease glucose levels, body weight, and ALT/AST levels at a dose of 25mg ED subQ over 4 weeks.

Human Study (ref: MOTS-c Human Study Extrapolation):

Found correlations between MOTS-c levels and insulin sensitivity in humans

"MOTS-c is hypothesized to be involved in the longevity of the Japanese" (ref: MOTS-c Human Study Extrapolation)

"The findings supported a role for MOTS-c as an endocrine-like peptide influencing insulin signaling pathways. The authors proposed that mitochondrial-derived peptides could serve as metabolic regulators and potential therapeutic targets for conditions such as obesity, insulin resistance, and type 2 diabetes. This study helped establish MOTS-c’s relevance in human metabolic physiology and inspired further investigations into its mechanisms and clinical applications."

"In the older cohort, higher MOTS-c levels were associated with improved muscle quality, based on maximal leg-press load relative to thigh cross-sectional area."

"The Phase 1b study was a randomized, double-blind, placebo-controlled evaluation of a 25 mg dose of CB4211 (MOTS-c analogue) given once daily by subcutaneous injection for four weeks in 20 obese subjects with nonalcoholic fatty liver disease (NAFLD)." (ref: Human Trial 1b of MOTS-c analogue results)

"Evaluation of the exploratory pharmacodynamic endpoints from the Phase 1b stage of the study comparing CB4211 to placebo demonstrated robust and significant reductions in key biomarkers of liver damage, ALT and AST, a significant decrease in glucose levels, and a trend towards lower body weight after four weeks of treatment." (ref: Human Trial 1b of MOTS-c analogue results)

Bottom Line for Fitness (Backed by Science)

MOTS-c is one of the most promising emerging peptides for:

Fat loss support

Endurance and performance

Metabolic efficiency

Healthy aging

Liver and organ support

Alzheimer's treatment

But right now, the science while exciting, is still early-stage and human clinical validation is limited. (ref: MOTS-c Human Study Extrapolation)

EVO members MOTS-c doses, anecdotal feedback and further research requests:

Quick cruise around EVO led me to these members and their doses with some anecdotal feedback

Please provide any reviews you wish to contribute include timing and reconstitution method that works best (ie: 2ml in 40mg vial, etc)

Please update your current dose of MOTS-c including your own quote on your thoughts about this peptide

MOTS-c is a bit of a niche peptide and those that run it usually know another member that does; I'd ask that you please tag as many EVO brothers or sisters you know that are running MOTS-c so that they can contribute to this research

If you have or are currently running MOTS-c and were not tagged below please add yourself to this community research thread by replying and I will add you to the list

Please add any additional research on MOTS-c to add to this research article including anecdotal experieces

@Allupfromhere 2mg ED “Mots c is one of my favs” "Mots c is like and endless fuel tank, i couldnt believe the stability in energy it has given me. Top 3 in my ranks for sure" You can follow his log here Allupfromhere's 2026 Growth Cycle Log - Team R&D
@San Andreas 2mg ED You can follow his log here San Andreas' 2026 Cycle Log powered by @War Born Peptides
@Darth_Vader 5mg e3d You can follow his log here Darth Vader's 2026 Cycle Log
@Demon_throne 14mg weekly (ie: 2mg ED) "The bread and butter of my mito stack." You can follow his log here Demon_Throne's 2026 Cycle Log powered by @Unitedau
@jwright1275 4mg e3d You can follow his log here jwright1275's 2026 Cycle Log
@G-Merc 80 1mg ED “I love MOTS-C and have found that SS31 pairs perfectly with it, just clean feelilng energy boost.” You can follow his log here G-Merc 80's 2026 Cycle Log
@Shakey 1mg ED You can follow his log here Shakey's 2026 Cycle Log
@HarleyGuy 1mg ED You can follow his log here HarleyGuy's 2026 Cycle Log powered by @biogenix
@Dreamer 5mg Pre-Bed “I have shared around the place how I use MOTS C, I take mine in the evening with my large intake of carbs, I have seen a massive improvement in fasting BGL in the morning and in turn insulin sensititivty by doing this.
I think peptides can be used in numerous different ways and applications once you understand what they do to YOUR body because they do seem to be highly individual, play around with them and monitor with data, they are incredibly useful for alot of things” You can follow his log hereDreamer's 2026 Cycle Log
@RogueCherry 1mg ED You can follow her log here RogueCherry's 2026 Reta/HGH Log
@Freakfitness 1mg ED You can follow his log here FreakFitness' 2026 Cycle Log
@Eveflorence 2mg ED “Found this worked amazingly well. Noticeable increases in energy, motivation, endurance and overall feeling of well-being. I want to have another run of it soon.” You can follow his log hereEveflorence's 2026 Cycle Log
@Doctakay 1mg pwo You can follow his log here Doctakay's 2026 Cycle Log
@jenkemj 2mg ED You can follow his log here jenkemj's 2026 Cycle Log powered by @Prymal
@willipede 1mg ED You can follow his log here willipede's 2026 Cycle Log
@Made_Man 2mg ED You can follow his log here Made_Man's 2026 Cycle Log
@TheOne1 10mg ED “So far my experience has been positive and i like the extra efficiency it has brought into my workouts. Also I have noticed a bit extra pump too. I'm looking forward to week 2 on this and seeing what else it can unlock as I use it more.” You can follow his log hereTheOne1's 2026 Cycle Log
@madcap71 2mg pwo You can follow his log heremadcap71's 2026 Cycle Log
@Flash_is_Fast 1mg pwo You can follow his log here Flash_is_Fast's 2026 Cycle Log
@fatboy999 1mg ED You can follow his log here Fatboy999's 2026 Cycle Log

Thanks for reading,
HarleyGuy

SLU-PP-332 Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast
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@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @BeMe @Farmboy @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @toddthelineman @Allupfromhere @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules

Awesome write up @HarleyGuy! thank you

LevButlerov · Mar 31, 2026

teddy_vio said:
It didn't work for me either, didn't notice anything at all from it. I have 9 more vials of it sitting in the freezer, maybe I'll try again someday.

what dose did you use? @teddy_vio

HarleyGuy · Mar 31, 2026

ceo said:
Bros, that's true for sure. I'm helping one of the dudes I work with. He is really out of shape; he wanted to get into better shape. He's starting to eat good; I'm proud of him.

I love this bro! Helping the world starts with one brother at a time!

HarleyGuy · Apr 1, 2026

Demon_throne said:
I will also be pushing up to 4mg daily at some point (I'll keep you updated for when I do, do this) just need to get myself a little extra stock so I can trial it for a while with out worrying about running out to see if there is much of a difference

Awesome bro drop us a line here when you start at 4mg and then a review after too. You will help a lot with some feedback on this dose. The most I've tried so far is 2mg.

HarleyGuy · Apr 1, 2026

Tiger Salman Khan said:
Good info....thanks for sharing......

Thanks brother @Tiger Salman Khan! It's great to give back.

HarleyGuy · Apr 1, 2026

teddy_vio said:
It didn't work for me either, didn't notice anything at all from it. I have 9 more vials of it sitting in the freezer, maybe I'll try again someday.

What dose did you run and were you basing this on cardio or just lifting? I'd love for you to give us a full review bro.

HarleyGuy · Apr 1, 2026

G-Merc 80 said:
Awesome write up @HarleyGuy! thank you

Thanks @G-Merc 80 I hope it helped!

Demon_throne · Apr 1, 2026

teddy_vio said:
It didn't work for me either, didn't notice anything at all from it. I have 9 more vials of it sitting in the freezer, maybe I'll try again someday.

How did you run it tho there are many ways

ceo · Apr 1, 2026

HarleyGuy said:
I love this bro! Helping the world starts with one brother at a time!

bros yesterday i caught him sneaking a twinky into his tool box lol

toddthelineman · Apr 1, 2026

ceo said:
bros yesterday i caught him sneaking a twinky into his tool box lol

It all starts at the store right? I try to not even go into stores. Especially convenience stores. I have the cravings and appetite of someone twice my size. So you just don’t buy the stuff, then you don’t have it at home. But families will sabotage you. It’s hard to get everyone on board. Usually impossible.

HarleyGuy · Apr 1, 2026

ceo said:
bros yesterday i caught him sneaking a twinky into his tool box lol

Haha we got a Gomar Pyle in the house!

HarleyGuy · Apr 1, 2026

toddthelineman said:
It all starts at the store right? I try to not even go into stores. Especially convenience stores. I have the cravings and appetite of someone twice my size. So you just don’t buy the stuff, then you don’t have it at home. But families will sabotage you. It’s hard to get everyone on board. Usually impossible.

Very good point. We are a small % of the population especially now

Flash_is_Fast · Apr 1, 2026

toddthelineman said:
It all starts at the store right? I try to not even go into stores. Especially convenience stores. I have the cravings and appetite of someone twice my size. So you just don’t buy the stuff, then you don’t have it at home. But families will sabotage you. It’s hard to get everyone on board. Usually impossible.

Families - my wife loves mexican food and my daughter has my sweet tooth (shes a rail - genetics). Man - the struggle is real.

I've thought about kicking them out, but apparently thats bad form. Society these days.

toddthelineman · Apr 1, 2026

Flash_is_Fast said:
Families - my wife loves mexican food and my daughter has my sweet tooth (shes a rail - genetics). Man - the struggle is real.

I've thought about kicking them out, but apparently thats bad form. Society these days.

I grew up on authentic Mexican food. It can be done fairly healthy I think

teddy_vio · Apr 1, 2026

Demon_throne said:
How did you run it tho there are many ways

I'll be honest it was a few months ago and I can't remember. I know I researched dosages/schedules and think I basically went middle of road. I was taking it in mornings, and was just hoping for less mid-day fatigue. I just never noticed any effect at all. I'll prob try again at some point.
Tirz, on the other hand, is a fucking miracle drug.

Demon_throne · Apr 1, 2026

teddy_vio said:
I'll be honest it was a few months ago and I can't remember. I know I researched dosages/schedules and think I basically went middle of road. I was taking it in mornings, and was just hoping for less mid-day fatigue. I just never noticed any effect at all. I'll prob try again at some point.
Tirz, on the other hand, is a fucking miracle drug.

Some protocols are as low as 500mcg eod.

HarleyGuy · Apr 1, 2026

teddy_vio said:
I'll be honest it was a few months ago and I can't remember. I know I researched dosages/schedules and think I basically went middle of road. I was taking it in mornings, and was just hoping for less mid-day fatigue. I just never noticed any effect at all. I'll prob try again at some point.
Tirz, on the other hand, is a fucking miracle drug.

I find if you're not active you don't notice much, if you're out and about it gives you lots of pep in your step and you feel more 'on'.

G-Merc 80 · Apr 2, 2026

teddy_vio said:
I'll be honest it was a few months ago and I can't remember. I know I researched dosages/schedules and think I basically went middle of road. I was taking it in mornings, and was just hoping for less mid-day fatigue. I just never noticed any effect at all. I'll prob try again at some point.
Tirz, on the other hand, is a fucking miracle drug.

I found that using 1mg daily worked well BUT if my calories were too low it almost worked in reverse or I wouldnt notice it; where I found it really shined was good diet, rest and paired with SS31, can go a whole day without a crash and reaching for a Monster Ultra

OwEv751 · Apr 2, 2026

I'm currently experiencing immune sensitisation to Mots-c and body is triggering a strong inflammatory response. Basically even 0.5mg injection (10mg/2ml) is still causing red swollen lumps. Currently running ketotifen, trying to time antihistamine to the shot and good dose of vitamin C.

Currently on PCT so I'm off AAS and only running hcg/hmg until tomorrow. Maybe the clue's in the bloods?

teddy_vio · Apr 2, 2026

OwEv751 said:
I'm currently experiencing immune sensitisation to Mots-c and body is triggering a strong inflammatory response. Basically even 0.5mg injection (10mg/2ml) is still causing red swollen lumps. Currently running ketotifen, trying to time antihistamine to the shot and good dose of vitamin C.

Currently on PCT so I'm off AAS and only running hcg/hmg until tomorrow. Maybe the clue's in the bloods?

Are you using any other peptides, and if yes, same BAC water with them and no reaction? Did you use BAC water from supplier, or real Hospira? I know its kind of a generic answer, but most seem to think that a lot of the issues are due to bad BAC water...

Community Research MOTS-C Peptide - Overview, Dosing, and Reviews

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What Is MOTS-c Peptide? (And Why We Are Paying Attention)​

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What Is MOTS-c Peptide? (And Why We Are Paying Attention)​

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What Is MOTS-c Peptide? (And Why We Are Paying Attention)​

3. Better Muscle Energy Utilization​

Bottom Line for Fitness (Backed by Science)​

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)

What Is MOTS-c Peptide? (And Why We Are Paying Attention)

3. Better Muscle Energy Utilization

Bottom Line for Fitness (Backed by Science)