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Community Research SLU-PP-332 Peptide - Administration Routes, Dosing, and Reviews

Threads marked with the 'Community Research' prefix involve ongoing research, high-quality logs, or in-depth community discussions backed by experience, data, or expert input.
HarleyGuy

HarleyGuy

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SLU-PP-332 PEPTIDE (a.k.a.: "SLUPP")
A readable layman's overview
Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC11584170/#S2

<<updated 01Jun2026>>


11Mar SLU-PP-332.webp

11Mar SLU-PP-332.webp
​


What is SLU-PP-332?

SLU-PP-332 is a non-selective and synthetic estrogen-related receptor (ERR) agonist (ERRα, ERRβ, and ERRγ) that acts as an "exercise mimetic". It may sound like it BUT this has nothing to do with estrogen nor does it bind to the estrogen receptor causing an increase in e2 levels. By activating these ERR receptors, it boosts metabolism, increases fatty acid oxidation, and improves muscle endurance in rodents, mimicking the effects of exercise. Dramatic results were observed in rodents even on a high calorie diet with little to no additional exercise.

Does it do this in humans? And at what dose? Time will tell as we ourselves become the 'rodents'


Properties of this peptide - knowns and unknowns as it relates to us:
  • It is hydrophobic (is not soluble in water)
  • Not soluble in bacteriostatic water, acetic acid, sterile water, etc
  • It is NOT (EVER!) to be reconstituted with DMSO and used for subQ injection (I have no problem with a blanket statement like this about injecting DMSO) and don't let anyone convince you otherwise
  • If taken orally in humans, the bioavailability is still up for debate and there is no human data
  • If taken subQ in humans, via reconstitution, the carrier required is still up for debate however it ought not be any of the above carriers


Routes of Administration in rodents:
  • intraperitoneal (big word!): this means it was injected into the stomach cavity


Routes of Administration in humans:

Oral: Due to it's hydrophobic nature it has been being used in enteric coated capsules to help survive the stomach and mitigate compound breakdown which leads to reduced bioavailability

SubQ Injection: Currently the only known anecdotal results among BB'ers who are properly NOT reconstituting this in any form of water are reconstituting it with filtered MCT oil for subQ injection


Dosaging in rodents:
  • "We observed no overt toxicity in mice administered SLU-PP-332 (50 mg/kg b.i.d., i.p.) for 10 days which is consistent with the normal complete blood count and electrolyte levels" - PubMed Study Nov2024
  • b.i.d. means twice per day. This means rodents were given 100mg/kg ED


Dosaging in humans:

Our brothers on EVO have all found themselves falling into different schools of thought. Oral vs. SubQ? Low dose vs. high dose?
Here are some examples of EVO members and their current dosages with the range difference:

EVO members' SLUPP dosing ranges: 250mcg - 150mg
<<updated 01Jun2026>>




Results found in rodents:
  • In summary, activation of ERRα by SLU-PP-332 as an exercise mimetic induces an acute aerobic exercise program that leads to an array of physiological adaptations that are associated with exercise, including increased oxidative fibers in a muscle, increased fatty acid oxidation, and enhanced exercise endurance.
  • Translation in layman's terms: In rodents. administration of SLU-PP-332 acts like the body has undergone aerobic exercise and increases oxidative fibers in muscle allowing for an increase in mitochodria and capillaries, lipolysis, and aerobic endurance.


Community Research Needed:

Let's please discuss all of the above as it relates to humans as a research thread to provide some anecdotal data to SLU-PP-332 in humans. What were your effects? Side effects? Dosages? Administration route? Should it be cycled?

Is there any doubt on the clinical data in rodents as it applies to humans? What can we add to what is already known in this community research thread?

I will discuss my intentions with it in my log as well as here.


Thanks for reading,
HarleyGuy 💀


Research Participants:
@LevButlerov @trenAMP @Nood @RawCutlery @liftedlivingwithlegacy @BigVelvetG @MarkNV @eazy_ @Neuro @US-pharmacies
@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @Grumpy @Demon_throne @R.AP @rizzlekdizzle @Pigsy @Wookie @AusMade @BeMe @Shakey @kcates @toddthelineman @ROIDDERS @2Thick @Ulter @ballin2504 @Mobster @stevesmi @kcates @Eddie Haskell @eazy_ @CladHQ @waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle @Freki @RawCutlery @Flash_is_Fast @Warthog61 @Yeahnah @Dumptruck @Nickh27 @Katsumi
Canadian EVO Loggers:
@BeMe @haldencapital @VerticallyChallenged @XxDennxX @Farmboy @Shakey @Lonewolf112 @frackz @WolfOfRoidStreet @nattybydesign @moreReps21 @JuiceyBrucey007 @zucchini @leaperfr @MTB @Kevdawg @IronCrusader33 @BritishCanadian @teddy_vio @Kevdawg @wye @stevedude19 @SomebodyDope @EL blueberry @GreatWhiteNorth8 @BertaBoy
MODS, et al: @BeMe @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ @Pigsy @s.gentz @stevesmi @toddthelineman @Allupfromhere @ROIDDERS @ceo @2Thick @Ulter
Podcast VIP's: @Eddie Haskell @LevButlerov @Mobster @Noah Wixx @Npcclassicphysique champ@Pigsy@s.gentz@toddthelineman@Allupfromhere @HarleyGuy @Dreamer @Freki @waggat @Yuri @rizzlekdizzle @MarkNV @BeMe @Farmboy @floridaman1984 @kcates @MarshMonsta @Wheels85 @TAGII @Jigglybuff @R.AP @Pat7x @SPOONMAN366 @RoySimpson @PrinceDaddy @stevesmi @Coolguy @liftedlivingwithlegacy @3xCharm @waggat @Kopite67 @madcap71 @Ohdamn @Swcc @Mr.liftz @waggat @Iron_rose1 @BigVelvetG @Demon_throne @codezz @Farmboy @Bigdan85 @Kopite67 @Nasser1997o2 @b1ak @Corn Gromwell @satxbber @BIGRED_ @rayray01 @fatboy999 @Lipster @Tankie @kamsikazee @Trenhead3cc @Robules @RedNeck @Acerico34 @Warthog61 @James Creeper @Simoneme @Robarock @CookieBaah @Struman @zucchini @Struman @CookieBaah @Robarock @Grumpy @catdadironman @wye @Asthetek23 @electus @Davie_Rich19 @Kopite67
Interested Participants:
@Panda22 @Sheshredz
 
Last edited:
HarleyGuy said:
SLU-PP-332 PEPTIDE (a.k.a.: "SLUPP")
A readable layman's overview
Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC11584170/#S2


View attachment 197230
View attachment 197248​


What is SLU-PP-332?

SLU-PP-332 is a non-selective and synthetic estrogen-related receptor (ERR) agonist that acts as an "exercise mimetic". It may sound like it BUT this has nothing to do with estrogen nor does it bind to the estrogen receptor causing an increase in e2 levels. By activating these ERR receptors, it boosts metabolism, increases fatty acid oxidation, and improves muscle endurance in rodents, mimicking the effects of exercise. Dramatic results were observed in rodents even on a high calorie diet with little to no additional exercise.

Does it do this in humans? And at what dose? Time will tell as we ourselves become the 'rodents'


Properties of this peptide - knowns and unknowns as it relates to us:
  • It is hydrophobic (is not soluble in water)
  • Not soluble in bacteriostatic water, acetic acid, sterile water, etc
  • It is NOT (EVER!) to be reconstituted with DMSO and used for subQ injection (I have no problem with a blanket statement like this about injecting DMSO) and don't let anyone convince you otherwise
  • If taken orally in humans, the bioavailability is still up for debate and there is no human data
  • If taken subQ in humans, via reconstitution, the carrier required is still up for debate however it ought not be any of the above carriers


Routes of Administration in rodents:
  • intraperitoneal (big word!): this means it was injected into the stomach cavity


Routes of Administration in humans:

Oral: Due to it's hydrophobic nature it has been being used in enteric coated capsules to help survive the stomach and mitigate compound breakdown which leads to reduced bioavailability

SubQ Injection: Currently the only known anecdotal results among BB'ers who are properly NOT reconstituting this in any form of water are reconstituting it with filtered MCT oil for subQ injection


Dosaging in rodents:
  • "We observed no overt toxicity in mice administered SLU-PP-332 (50 mg/kg b.i.d., i.p.) for 10 days which is consistent with the normal complete blood count and electrolyte levels" - PubMed Study Nov2024
  • b.i.d. means twice per day. This means rodents were given 100mg/kg ED


Dosaging in humans:

Our brothers on EVO have all found themselves falling into different schools of thought. Oral vs. SubQ? Low dose vs. high dose?
Here are some examples of EVO members and their current dosages:



Results found in rodents:
  • In summary, activation of ERRα by SLU-PP-332 as an exercise mimetic induces an acute aerobic exercise program that leads to an array of physiological adaptations that are associated with exercise, including increased oxidative fibers in a muscle, increased fatty acid oxidation, and enhanced exercise endurance.
  • Translation in layman's terms: In rodents. administration of SLU-PP-332 acts like the body has undergone aerobic exercise and increases oxidative fibers in muscle allowing for an increase in mitochodria and capillaries, lipolysis, and aerobic endurance.


Community Research Needed:

Let's please discuss all of the above as it relates to humans as a research thread to provide some anecdotal data to SLU-PP-332 in humans. What were your effects? Side effects? Dosages? Administration route? Should it be cycled?

Is there any doubt on the clinical data in rodents as it applies to humans? What can we add to what is already known in this community research thread?

I will discuss my intentions with it in my log as well as here.


Thanks for reading,
HarleyGuy
Click to expand...
This will be an interesting SLUPP thread lets see how it goes :D as we upgrade the info @HarleyGuy thank you for sharing this!

@Allupfromhere @Pigsy @Dreamer @Freki @Grumpy
@waggat @Trenhead3cc @Kopite67 @codezz @Yuri @MarkNV @rizzlekdizzle
 
Here's some quotes from our EVO brothers' logs of when they were (or currently are) using SLU-PP-332 with their dosages as well:


Allupfromhere said:
50mg SLU-PP-332 (split)
Click to expand...

fingers86 said:
slu 50mg entric caps morning and lunch
Click to expand...

liftedlivingwithlegacy said:
SLU-PPP 1 mg daily
Click to expand...

roguepineapple said:
@CladHQ's slu has been a great compound to work with, and it has seriously helped with giving me a boost in my results. Obviously, not the main driver, but an awesome addition to a stack when everything is dialled in.
Click to expand...

CladHQ said:
I am personally starting the 40mg caps on monday. Excited to try at higher dose. I had great results at 20mg, so will push to 40mg to start and then 80mg.
Click to expand...

CladHQ said:
Great to hear the positive feedback on the 20mg SLU.
Click to expand...
Mentioned by @CladHQ to @roguepineapple ⬆️


LH5515 said:
Mitochondrial Stack
I brought over only some of the stack to start while away. I have been running @CladHQ Mots C 2mg / 5 x per week, SLU 5mg Caps (20mg pre workout) & L-Carnitine 600mg pre-workout for the past 3 weeks.
Click to expand...

justapotato said:
50mg Slupp332 Daily
Click to expand...


@liftedlivingwithlegacy @LH5515 @justapotato @Allupfromhere @roguepineapple @fingers86 @CladHQ
 
Last edited:
HarleyGuy said:
Yes let's bring in @Allupfromhere for some organic chemistry tomfoolery details!! I didn't have the bandwidth left to get into it after a full shift on EVO but I think he's waking up right about now :p
Click to expand...
for sure this will be a good thread coming into this month :D will be adding more to it as well @HarleyGuy
 
kcates said:
How you going to test if you get complete or incomplete dissolution with MCT oil?
Click to expand...
Impossible unless I win the lottery and buy $250,000 worth of scientific equipment to test.

However, not unlike how I trust the science and research that my GH isn't hydrophobic and will dissolve in bac water, same with the NAD+, GH, and Reta you posted on 03Mar I'm assuming you trust the science that that is soluble in bac water? I trust the science that SLUPP in fact IS hydrophobic and needs an oil based carrier like what DMSO is and certainly not any type of water. The reason DMSO is suggested vs. bac water all this time is that DMSO (which isn't technically an oil) is quite viscous and therefore may make SLUPP soluble but at very great risk by injecting this carrier. A thin less viscous oil, on the other hand, like MCT oil ought to yield much more certain solubility than water when reconstituting a hydrophobic peptide.
 
What are anyone's thoughts on adding in some EO with the MCT to further assist solubility? @LevButlerov
EO is technically a solvent but has much broader and safer use in the context of injection subQ and IM and is in a lot of our approved sources higher concentration gear.

@Shakey I think we were talking about this and it was your idea?
 
HarleyGuy said:
Are yours capsules @rizzlekdizzle? Mine are tablets so they're garbage imo
Click to expand...
some people are selling the powder too in a bag. So that would just be eaten or 'dissolved' in water right?
Mine are tabs but seem to have a a shine to em - so perhaps a coating?
 
Great research thread @HarleyGuy. Yeah, EO is technically a solvent. Its mainly used to reduce viscosity and increase dissolution of a compound in carrier oils, especially with compounds in higher concentrations. So adding EO to the MCT would definitely help. There are even (though very rare) instances of EO as 100% the carrier for a medication in humans (that are allergic to the carrier oils that some meds come in) and animals.
 
rizzlekdizzle said:
some people are selling the powder too in a bag. So that would just be eaten or 'dissolved' in water right?
Mine are tabs but seem to have a a shine to em - so perhaps a coating?
Click to expand...
Yes buying raw SLUPP powder is one way it's being done. What most bros are doing is then purchasing their own enteric capsules and buying micro-measuring spoons and filling the capsules themselves. Thing is, a 1000mcg spoon is hard to gauge whether it's half full if you want to dose 500mcg. :p If you're in the camp that more is better then the 5-10mg spoons will suffice but then you better make darn sure you have the right size capsules.
 
Shakey said:
Great research thread @HarleyGuy. Yeah, EO is technically a solvent. Its mainly used to reduce viscosity and increase dissolution of a compound in carrier oils, especially with compounds in higher concentrations. So adding EO to the MCT would definitely help. There are even (though very rare) instances of EO as 100% the carrier for a medication in humans (that are allergic to the carrier oils that some meds come in) and animals.
Click to expand...
Ok so we might be on to something here then. There's no doubt in my mind it can't be a water based solution and that solvent in fact IS best (like DMSO) however injecting it is a non-starter. I happen to know that I have zero reaction to EO so this might be a pivot to my MCT-only idea.
 
HarleyGuy said:
Yes buying raw SLUPP powder is one way it's being done. What most bros are doing is then purchasing their own enteric capsules and buying micro-measuring spoons and filling the capsules themselves. Thing is, a 1000mcg spoon is hard to gauge whether it's half full if you want to dose 500mcg. :p If you're in the camp that more is better then the 5-10mg spoons will suffice but then you better make darn sure you have the right size capsules.
Click to expand...
I've also got raw powder making up caps
Its a bit of a pain to make the caps up but works well
 
HarleyGuy said:
Ok so we might be on to something here then. There's no doubt in my mind it can't be a water based solution and that solvent in fact IS best (like DMSO) however injecting it is a non-starter. I happen to know that I have zero reaction to EO so this might be a pivot to my MCT-only idea.
Click to expand...
The only problem as it is a solvent, some dont have a good reaction. So a heavy EO ratio wont be good for everyone, but a few experiments to find a good dissolution ratio would be great.
 
rizzlekdizzle said:
Mine are tabs but seem to have a a shine to em - so perhaps a coating?
Click to expand...
Hmmm, this is a tough one. See mine are clearly just pill pressed tabs and cheap. Yours mayyyybe could have some sort of enteric dip on them which would be pretty fancy and I don't think would work as well as good capsules designed to withstand the stomach.

rizzlekdizzle said:
some people are selling the powder too in a bag. So that would just be eaten or 'dissolved' in water right?
Click to expand...
They could eat it or 'dissolve' it and drink it but they'd be wasting it this way. It would get instantly chewed up into literally more than a million pieces in the gut and not be active at all.
 
Grumpy said:
I've also got raw powder making up caps
Its a bit of a pain to make the caps up but works well
Click to expand...
And they're enteric coated capsules? I can't remember if you're running it now have you started and what's your dose? Effects/side effects? I'll add you to the original post if this is the case bro.
 
Shakey said:
The only problem as it is a solvent, some dont have a good reaction. So a heavy EO ratio wont be good for everyone, but a few experiments to find a good dissolution ratio would be great.
Click to expand...
I know for a fact I don't react at all to EO so this won't be an issue for me. I shot a message to @Fusion Canada to ask what the ratio of his EO is in his high concentration gear because I've used them all and I get zero reaction or PIP from it whatsoever.
 
HarleyGuy said:
And they're enteric coated capsules? I can't remember if you're running it now have you started and what's your dose? Effects/side effects? I'll add you to the original post if this is the case bro.
Click to expand...
Yes enteric caps 10mg twice per day first few days felt a bit sluggish definitely sweating a lot more than usual im around 3 to 4 weeks in now and dont feel any side effects except for the extra sweating and maybe a slight increase in cardio activity
 
HarleyGuy said:
I know for a fact I don't react at all to EO so this won't be an issue for me. I shot a message to @Fusion Canada to ask what the ratio of his EO is in his high concentration gear because I've used them all and I get zero reaction or PIP from it whatsoever.
Click to expand...
Great. for DIY home testing, perhaps letting a fresh reconn sit for 72hrs for crystals to settle and aggregate. You can then filter and a see if pressure used while filtering is similar to filtering an already fully dissolved compound from one of the sources here. that would atleast give an idea.
 
Grumpy said:
Yes enteric caps 10mg twice per day first few days felt a bit sluggish definitely sweating a lot more than usual im around 3 to 4 weeks in now and dont feel any side effects except for the extra sweating and maybe a slight increase in cardio activity
Click to expand...
You sir have been added to the original post. Thanks for hopping in here!
 
Shakey said:
Great. for DIY home testing, perhaps letting a fresh reconn sit for 72hrs for crystals to settle and aggregate. You can then filter and a see if pressure used while filtering is similar to filtering an already fully dissolved compound from one of the sources here. that would atleast give an idea.
Click to expand...
Another good idea for real, however it sounds highly annoying to do :ROFLMAO:
 
Mobster said:
Feedback here will help for sure
Click to expand...
Feedback agreed. Convincing scientific and anecdotal data in humans isn't out there yet except for some well thought out arguments. One of the best arguments I've heard for not going high dose was by Dave Crosland and Dr. Dean St Mart on the Think Big Podcast. @Mobster knows Crosland and probably outlifted him at some point :ROFLMAO:. They also explain why the 'sweating' side effect of it means your dose is way too high and it's acting almost like an uncoupler at that point.
 
I've found no side effects running 80mg daily it's been great I'm taking it as 4x 20mg caps currently (because that's how I made them) but once there used up I'm switching to 3x 30mg caps.

I fell it was truly the one additional compound that helped take my cut as far as it did in a short time, if I could go back I would have implemented it from the get go!
 
HarleyGuy said:
SLU-PP-332 PEPTIDE (a.k.a.: "SLUPP")
A readable layman's overview
Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC11584170/#S2


View attachment 197230
View attachment 197248​


What is SLU-PP-332?

SLU-PP-332 is a non-selective and synthetic estrogen-related receptor (ERR) agonist that acts as an "exercise mimetic". It may sound like it BUT this has nothing to do with estrogen nor does it bind to the estrogen receptor causing an increase in e2 levels. By activating these ERR receptors, it boosts metabolism, increases fatty acid oxidation, and improves muscle endurance in rodents, mimicking the effects of exercise. Dramatic results were observed in rodents even on a high calorie diet with little to no additional exercise.

Does it do this in humans? And at what dose? Time will tell as we ourselves become the 'rodents'


Properties of this peptide - knowns and unknowns as it relates to us:
  • It is hydrophobic (is not soluble in water)
  • Not soluble in bacteriostatic water, acetic acid, sterile water, etc
  • It is NOT (EVER!) to be reconstituted with DMSO and used for subQ injection (I have no problem with a blanket statement like this about injecting DMSO) and don't let anyone convince you otherwise
  • If taken orally in humans, the bioavailability is still up for debate and there is no human data
  • If taken subQ in humans, via reconstitution, the carrier required is still up for debate however it ought not be any of the above carriers


Routes of Administration in rodents:
  • intraperitoneal (big word!): this means it was injected into the stomach cavity


Routes of Administration in humans:

Oral: Due to it's hydrophobic nature it has been being used in enteric coated capsules to help survive the stomach and mitigate compound breakdown which leads to reduced bioavailability

SubQ Injection: Currently the only known anecdotal results among BB'ers who are properly NOT reconstituting this in any form of water are reconstituting it with filtered MCT oil for subQ injection


Dosaging in rodents:
  • "We observed no overt toxicity in mice administered SLU-PP-332 (50 mg/kg b.i.d., i.p.) for 10 days which is consistent with the normal complete blood count and electrolyte levels" - PubMed Study Nov2024
  • b.i.d. means twice per day. This means rodents were given 100mg/kg ED


Dosaging in humans:

Our brothers on EVO have all found themselves falling into different schools of thought. Oral vs. SubQ? Low dose vs. high dose?
Here are some examples of EVO members and their current dosages:



Results found in rodents:
  • In summary, activation of ERRα by SLU-PP-332 as an exercise mimetic induces an acute aerobic exercise program that leads to an array of physiological adaptations that are associated with exercise, including increased oxidative fibers in a muscle, increased fatty acid oxidation, and enhanced exercise endurance.
  • Translation in layman's terms: In rodents. administration of SLU-PP-332 acts like the body has undergone aerobic exercise and increases oxidative fibers in muscle allowing for an increase in mitochodria and capillaries, lipolysis, and aerobic endurance.


Community Research Needed:

Let's please discuss all of the above as it relates to humans as a research thread to provide some anecdotal data to SLU-PP-332 in humans. What were your effects? Side effects? Dosages? Administration route? Should it be cycled?

Is there any doubt on the clinical data in rodents as it applies to humans? What can we add to what is already known in this community research thread?

I will discuss my intentions with it in my log as well as here.


Thanks for reading,
HarleyGuy
Click to expand...
i don't think this is something that i need but i know a lot of people who could use it.
 
Demon_throne said:
I've found no side effects running 80mg daily it's been great I'm taking it as 4x 20mg caps currently (because that's how I made them) but once there used up I'm switching to 3x 30mg caps.

I fell it was truly the one additional compound that helped take my cut as far as it did in a short time, if I could go back I would have implemented it from the get go!
Click to expand...
Thanks for hopping onto the research thread @Demon_throne! You have been added to the original post with log and dosing for SLUPP!

This is some great feedback about SLUPP. So no fatigue at those doses? I know @Allupfromhere had some crazy fatigue at 100mg but it was fleeting and only on a few occasions.
 
HarleyGuy said:
Thanks for hopping onto the research thread @Demon_throne! You have been added to the original post with log and dosing for SLUPP!

This is some great feedback about SLUPP. So no fatigue at those doses? I know @Allupfromhere had some crazy fatigue at 100mg but it was fleeting and only on a few occasions.
Click to expand...
I run it a little different then most, I normally have to pee about 2 hrs before I wake for the day so I have my first cap then, second upon waking fully for the day 3rd a few hours after and final one about 2 hrs before bed, I keep it away from my weight traning I've found it more effective.

I have a feeling it also depends on what else you are running as well. My set up will remain mostly the same for my upcoming growth phase where by keeping it out of my traning window I avoid it around insulin use

I know @R.AP is also running it during his prep
 
HarleyGuy said:
Impossible unless I win the lottery and buy $250,000 worth of scientific equipment to test.

However, not unlike how I trust the science and research that my GH isn't hydrophobic and will dissolve in bac water, same with the NAD+, GH, and Reta you posted on 03Mar I'm assuming you trust the science that that is soluble in bac water? I trust the science that SLUPP in fact IS hydrophobic and needs an oil based carrier like what DMSO is and certainly not any type of water. The reason DMSO is suggested vs. bac water all this time is that DMSO (which isn't technically an oil) is quite viscous and therefore may make SLUPP soluble but at very great risk by injecting this carrier. A thin less viscous oil, on the other hand, like MCT oil ought to yield much more certain solubility than water when reconstituting a hydrophobic peptide.
Click to expand...
From what I understand slu-pp-332 is a high polar molecule and needs another polar solvent to bond to and dissolve properly. Mct oil is a non polar oil and will bond and dissolve non polar oil. The basic rule of chemistry is like dissolves like. This is going against the basic rule of chemistry. That's why I trust other pepetides and compounds mixing with the solvents they mix with because they are following the rule for the ones that are not water soluble. The other ones u mention go with water because they are proven to be highly water soluble already. Slu-pp-332 has proven to not be water soluble so then u have to mix with a solvent. What about using a peg 300 or 400 have u looked into the science of using one of those. I think 400 mixes with saline. Then could be added to saline after the slu-pp is mixed with the peg-400 from what I understand.
 
Last edited:
kcates said:
From what I understand slu-pp-332 is a high polar molecule and needs another polar solvent to bond to and dissolve properly. Mct oil is a non polar oil and will bond and dissolve non polar oil. The basic rule of chemistry is like dissolves like. This is going against the basic rule of chemistry. That's why I trust other pepetides and compounds mixing with the solvents they mix with because they are following the rule for the ones that are not water soluble. The other ones u mention go with water because they are proven to be highly water soluble already. Slu-pp-332 has proven to not be water soluble so then u have to mix with a solvent. What about using a peg 300 or 400 have u looked into the science of using one of those. I think 400 mixes with saline. Then could be added to saline after the slu-pp is mixed with the peg-400 from what I understand.
Click to expand...
That's why I was asking about how u were looking to figure out if it dissolves well. I think the molecular level they won't bond then u won't have equal doses. U could have high dose one day and low doses another day and I hear to high of inject dose of slu-pp- 332 is not good.
 
HarleyGuy said:
SLU-PP-332 PEPTIDE (a.k.a.: "SLUPP")
A readable layman's overview
Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC11584170/#S2


View attachment 197230
View attachment 197248​


What is SLU-PP-332?

SLU-PP-332 is a non-selective and synthetic estrogen-related receptor (ERR) agonist that acts as an "exercise mimetic". It may sound like it BUT this has nothing to do with estrogen nor does it bind to the estrogen receptor causing an increase in e2 levels. By activating these ERR receptors, it boosts metabolism, increases fatty acid oxidation, and improves muscle endurance in rodents, mimicking the effects of exercise. Dramatic results were observed in rodents even on a high calorie diet with little to no additional exercise.

Does it do this in humans? And at what dose? Time will tell as we ourselves become the 'rodents'


Properties of this peptide - knowns and unknowns as it relates to us:
  • It is hydrophobic (is not soluble in water)
  • Not soluble in bacteriostatic water, acetic acid, sterile water, etc
  • It is NOT (EVER!) to be reconstituted with DMSO and used for subQ injection (I have no problem with a blanket statement like this about injecting DMSO) and don't let anyone convince you otherwise
  • If taken orally in humans, the bioavailability is still up for debate and there is no human data
  • If taken subQ in humans, via reconstitution, the carrier required is still up for debate however it ought not be any of the above carriers


Routes of Administration in rodents:
  • intraperitoneal (big word!): this means it was injected into the stomach cavity


Routes of Administration in humans:

Oral: Due to it's hydrophobic nature it has been being used in enteric coated capsules to help survive the stomach and mitigate compound breakdown which leads to reduced bioavailability

SubQ Injection: Currently the only known anecdotal results among BB'ers who are properly NOT reconstituting this in any form of water are reconstituting it with filtered MCT oil for subQ injection


Dosaging in rodents:
  • "We observed no overt toxicity in mice administered SLU-PP-332 (50 mg/kg b.i.d., i.p.) for 10 days which is consistent with the normal complete blood count and electrolyte levels" - PubMed Study Nov2024
  • b.i.d. means twice per day. This means rodents were given 100mg/kg ED


Dosaging in humans:

Our brothers on EVO have all found themselves falling into different schools of thought. Oral vs. SubQ? Low dose vs. high dose?
Here are some examples of EVO members and their current dosages:



Results found in rodents:
  • In summary, activation of ERRα by SLU-PP-332 as an exercise mimetic induces an acute aerobic exercise program that leads to an array of physiological adaptations that are associated with exercise, including increased oxidative fibers in a muscle, increased fatty acid oxidation, and enhanced exercise endurance.
  • Translation in layman's terms: In rodents. administration of SLU-PP-332 acts like the body has undergone aerobic exercise and increases oxidative fibers in muscle allowing for an increase in mitochodria and capillaries, lipolysis, and aerobic endurance.


Community Research Needed:

Let's please discuss all of the above as it relates to humans as a research thread to provide some anecdotal data to SLU-PP-332 in humans. What were your effects? Side effects? Dosages? Administration route? Should it be cycled?

Is there any doubt on the clinical data in rodents as it applies to humans? What can we add to what is already known in this community research thread?

I will discuss my intentions with it in my log as well as here.


Thanks for reading,
HarleyGuy
Click to expand...
Bros, this one I've been hearing about lately. A lot of the Aussie guys are using it.
 
HarleyGuy said:
SLU-PP-332 PEPTIDE (a.k.a.: "SLUPP")
A readable layman's overview
Ref: https://pmc.ncbi.nlm.nih.gov/articles/PMC11584170/#S2


View attachment 197230
View attachment 197248​


What is SLU-PP-332?

SLU-PP-332 is a non-selective and synthetic estrogen-related receptor (ERR) agonist that acts as an "exercise mimetic". It may sound like it BUT this has nothing to do with estrogen nor does it bind to the estrogen receptor causing an increase in e2 levels. By activating these ERR receptors, it boosts metabolism, increases fatty acid oxidation, and improves muscle endurance in rodents, mimicking the effects of exercise. Dramatic results were observed in rodents even on a high calorie diet with little to no additional exercise.

Does it do this in humans? And at what dose? Time will tell as we ourselves become the 'rodents'


Properties of this peptide - knowns and unknowns as it relates to us:
  • It is hydrophobic (is not soluble in water)
  • Not soluble in bacteriostatic water, acetic acid, sterile water, etc
  • It is NOT (EVER!) to be reconstituted with DMSO and used for subQ injection (I have no problem with a blanket statement like this about injecting DMSO) and don't let anyone convince you otherwise
  • If taken orally in humans, the bioavailability is still up for debate and there is no human data
  • If taken subQ in humans, via reconstitution, the carrier required is still up for debate however it ought not be any of the above carriers


Routes of Administration in rodents:
  • intraperitoneal (big word!): this means it was injected into the stomach cavity


Routes of Administration in humans:

Oral: Due to it's hydrophobic nature it has been being used in enteric coated capsules to help survive the stomach and mitigate compound breakdown which leads to reduced bioavailability

SubQ Injection: Currently the only known anecdotal results among BB'ers who are properly NOT reconstituting this in any form of water are reconstituting it with filtered MCT oil for subQ injection


Dosaging in rodents:
  • "We observed no overt toxicity in mice administered SLU-PP-332 (50 mg/kg b.i.d., i.p.) for 10 days which is consistent with the normal complete blood count and electrolyte levels" - PubMed Study Nov2024
  • b.i.d. means twice per day. This means rodents were given 100mg/kg ED


Dosaging in humans:

Our brothers on EVO have all found themselves falling into different schools of thought. Oral vs. SubQ? Low dose vs. high dose?
Here are some examples of EVO members and their current dosages:



Results found in rodents:
  • In summary, activation of ERRα by SLU-PP-332 as an exercise mimetic induces an acute aerobic exercise program that leads to an array of physiological adaptations that are associated with exercise, including increased oxidative fibers in a muscle, increased fatty acid oxidation, and enhanced exercise endurance.
  • Translation in layman's terms: In rodents. administration of SLU-PP-332 acts like the body has undergone aerobic exercise and increases oxidative fibers in muscle allowing for an increase in mitochodria and capillaries, lipolysis, and aerobic endurance.


Community Research Needed:

Let's please discuss all of the above as it relates to humans as a research thread to provide some anecdotal data to SLU-PP-332 in humans. What were your effects? Side effects? Dosages? Administration route? Should it be cycled?

Is there any doubt on the clinical data in rodents as it applies to humans? What can we add to what is already known in this community research thread?

I will discuss my intentions with it in my log as well as here.


Thanks for reading,
HarleyGuy
Click to expand...
this is some cool stuff. The mitochondria benefits alone make this stuff worth it. I'm not sure about the aerobic endurance though. I would definitely stick to GW with that but I like the other stuff it does.
 
I'm liking these types of threads. These new compounds that you millennials keep coming up with are very fascinating to me and this old man is definitely interested in hearing more about them but you know how it goes. I'm stuck in my old ways.
 
So ive looked into Human Equivalent Dose (HED) conversion and ive found some data and equations too convert different animal doses to humans based on Body Surface Area (BSA) which correlates better with metabolism and toxicity than body weight does.

BSA is used for dose translation to humans in preclinical studies by the FDA. for safe practice, they divive the HED by 10 for initail human trial dosing. The Km constant for a 60kg human is 37. Ill show the Km method using the human constant first. Then a formula for adjusting the Km factor for you personally based on your height (cm) and weight (kg) to find your BSA instead of the BSA based on the average human weight across the board for studies.

HED = Human Equivalent Dose
BSA = Body Surface Area
My weight in kg is 82.77kg
My height in cm is 191.77cm

Km Method
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/37)
HED = 50mg/kg x 0.081
HED = 4.05mg/kg
4.05mg x 82.77kg = 335mg

Mosteller BSA Formula - Finds your BSA
BSA (m²) = square root of ((height in cm × weight in kg) / 3600)
BSA (m²) = √ ((1911.77 x 82.77) / 3600)
BSA (m²) = √ (15872.8 / 3600)
BSA (m²) = √4.409
BSA (m²) = 2.099m²

Km Factor Adjustment - Finds your Km constant
Km = weight / BSA
Km = 82.77kg / 2.099m²
Km = 39.43

Km Method with My Constant
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/39.43)
HED = 50mg/kg x 0.076
HED = 3.8mg/kg
3.8mg x 82.77kg = 315mg

A 50mg/kg mouse dose is equivalent to a 315mg dose for me. Though different delivery method would effect this.

A Simple Practice Guide For Dose Conversion Between Animals And Humans
https://pmc.ncbi.nlm.nih.gov/articles/PMC4804402/ - FDA chart attached
FDA Guidance For Industry on Starting Dose In Human Trials
https://www.fda.gov/media/72309/download
 

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kcates said:
From what I understand slu-pp-332 is a high polar molecule and needs another polar solvent to bond to and dissolve properly. Mct oil is a non polar oil and will bond and dissolve non polar oil. The basic rule of chemistry is like dissolves like. This is going against the basic rule of chemistry. That's why I trust other pepetides and compounds mixing with the solvents they mix with because they are following the rule for the ones that are not water soluble. The other ones u mention go with water because they are proven to be highly water soluble already. Slu-pp-332 has proven to not be water soluble so then u have to mix with a solvent. What about using a peg 300 or 400 have u looked into the science of using one of those. I think 400 mixes with saline. Then could be added to saline after the slu-pp is mixed with the peg-400 from what I understand.
Click to expand...
This is a very convincing argument. I've been looking into using EO instead. What are your thoughts on Ethyl Oleate straight up?
 
Ulter said:
I'm liking these types of threads. These new compounds that you millennials keep coming up with are very fascinating to me and this old man is definitely interested in hearing more about them but you know how it goes. I'm stuck in my old ways.
Click to expand...
We love you Ulter haha!!
 
Shakey said:
So ive looked into Human Equivalent Dose (HED) conversion and ive found some data and equations too convert different animal doses to humans based on Body Surface Area (BSA) which correlates better with metabolism and toxicity than body weight does.

BSA is used for dose translation to humans in preclinical studies by the FDA. for safe practice, they divive the HED by 10 for initail human trial dosing. The Km constant for a 60kg human is 37. Ill show the Km method using the human constant first. Then a formula for adjusting the Km factor for you personally based on your height (cm) and weight (kg) to find your BSA instead of the BSA based on the average human weight across the board for studies.

HED = Human Equivalent Dose
BSA = Body Surface Area
My weight in kg is 82.77kg
My height in cm is 191.77cm

Km Method
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/37)
HED = 50mg/kg x 0.081
HED = 4.05mg/kg
4.05mg x 82.77kg = 335mg

Mosteller BSA Formula - Finds your BSA
BSA (m²) = square root of ((height in cm × weight in kg) / 3600)
BSA (m²) = √ ((1911.77 x 82.77) / 3600)
BSA (m²) = √ (15872.8 / 3600)
BSA (m²) = √4.409
BSA (m²) = 2.099m²

Km Factor Adjustment - Finds your Km constant
Km = weight / BSA
Km = 82.77kg / 2.099m²
Km = 39.43

Km Method with My Constant
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/39.43)
HED = 50mg/kg x 0.076
HED = 3.8mg/kg
3.8mg x 82.77kg = 315mg

A 50mg/kg mouse dose is equivalent to a 315mg dose for me. Though different delivery method would effect this.

A Simple Practice Guide For Dose Conversion Between Animals And Humans
https://pmc.ncbi.nlm.nih.gov/articles/PMC4804402/ - FDA chart attached
FDA Guidance For Industry on Starting Dose In Human Trials
https://www.fda.gov/media/72309/download
Click to expand...
Amazing work here @Shakey!! There's no doubt the HED will translate into a dose that high and your math is bang on. So it seems clinical trials would start with high doses (as they always seem to) and beyond.

Since we can't do intraperitoneal injection I'm hoping subQ comes close to equivalent vs. oral. Having said that I ain't going higher than 2mg subQ ED :ROFLMAO: and not 315mg... we'll leave that for the human trial survivors LOL.
 
@R.AP what's your current dose of SLUPP and how are you finding it?
 
Ulter said:
Love you more you tatted up Biker you. I would never let my daughter date you.
Click to expand...
Awe I take that as a compliment :love:. Give me the angry eyes and don't let me near her! :p
 
HarleyGuy said:
Amazing work here @Shakey!! There's no doubt the HED will translate into a dose that high and your math is bang on. So it seems clinical trials would start with high doses (as they always seem to) and beyond.

Since we can't do intraperitoneal injection I'm hoping subQ comes close to equivalent vs. oral. Having said that I ain't going higher than 2mg subQ ED :ROFLMAO: and not 315mg... we'll leave that for the human trial survivors LOL.
Click to expand...
FDA approved starting dose would be 33.5mg based on a 60kg human.
 
Shakey said:
FDA approved starting dose would be 33.5mg based on a 60kg human.
Click to expand...
Ya well I bet you can’t calculate a rodent IP injection conversion to human subQ. Take that!!
 
HarleyGuy said:
Ya well I bet you can’t calculate a rodent IP injection conversion to human subQ. Take that!!
Click to expand...
Looks like IP conversion to Sub Q should be the same. The difference though is the absorption rate, where IP injections absorb quicker and have a higher peak plasma level. The thing to watch out for is the difference in toxicity and effectiveness between species. That's why the FDA initial dose for human testing is 10%.
 
Shakey said:
So ive looked into Human Equivalent Dose (HED) conversion and ive found some data and equations too convert different animal doses to humans based on Body Surface Area (BSA) which correlates better with metabolism and toxicity than body weight does.

BSA is used for dose translation to humans in preclinical studies by the FDA. for safe practice, they divive the HED by 10 for initail human trial dosing. The Km constant for a 60kg human is 37. Ill show the Km method using the human constant first. Then a formula for adjusting the Km factor for you personally based on your height (cm) and weight (kg) to find your BSA instead of the BSA based on the average human weight across the board for studies.

HED = Human Equivalent Dose
BSA = Body Surface Area
My weight in kg is 82.77kg
My height in cm is 191.77cm

Km Method
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/37)
HED = 50mg/kg x 0.081
HED = 4.05mg/kg
4.05mg x 82.77kg = 335mg

Mosteller BSA Formula - Finds your BSA
BSA (m²) = square root of ((height in cm × weight in kg) / 3600)
BSA (m²) = √ ((1911.77 x 82.77) / 3600)
BSA (m²) = √ (15872.8 / 3600)
BSA (m²) = √4.409
BSA (m²) = 2.099m²

Km Factor Adjustment - Finds your Km constant
Km = weight / BSA
Km = 82.77kg / 2.099m²
Km = 39.43

Km Method with My Constant
HED = Animal Dose x (Animal Km / Human Km)
HED = 50mg/kg x (3/39.43)
HED = 50mg/kg x 0.076
HED = 3.8mg/kg
3.8mg x 82.77kg = 315mg

A 50mg/kg mouse dose is equivalent to a 315mg dose for me. Though different delivery method would effect this.

A Simple Practice Guide For Dose Conversion Between Animals And Humans
https://pmc.ncbi.nlm.nih.gov/articles/PMC4804402/ - FDA chart attached
FDA Guidance For Industry on Starting Dose In Human Trials
https://www.fda.gov/media/72309/download
Click to expand...
Dude thanks for putting this up. I like the nerdy stuff like this for my own knowledge.
 
kcates said:
Dude thanks for putting this up. I like the nerdy stuff like this for my own knowledge.
Click to expand...
Bro, then youll love my Syringe dead space and micro dosing article. i should be posting this weekend. its got a few algebra formulas in it along with dead volume measurements pulled from multiple studies and a technique referenced from a nursing textbook. its sitting at 2100 words, but ill be rewriting and removing a few redundant sections after changing how ill present math and examples.

i aimed for 150mg test weekly, but after measuring my generic pins deadspace with filtered water/food colouring and air locking to deliver dead volume residual waste, im at 234.5mg weekly. airlocking effectively turns a vial yield loss into an overdose by clearing the dead space of compound using a pocket of air during injection. the smaller the volume injected, the larger the vial yield loss.

youll love it. i even added pictures so @HarleyGuy can follow along. 🤣
 
HarleyGuy said:
This is a very convincing argument. I've been looking into using EO instead. What are your thoughts on Ethyl Oleate straight up?
Click to expand...
My first thought is it is mostly non polar and lipophilic but has one ester that gives it a little bit of polarity but is that going to be enough to bind it and keep it from dispersing and crystalizing possible. My first guess Is no because slupp is a highly polar molecole. I need to look into this one more before I can give a full thought on it. I am personally looking at now using peg 400 to mix it with first then mix it with sterile water. peg is pharmacitical and food grade approved by the FDA. I was talking with my supplier today about this idea because I told him I didn't want to use dmso and put that in my body and they said if I tried this instead of dmso and it crystalize they would send me a whole new batch of slu-pp for free.for being the Ginnie pig. I will look into the EO after dinner. I just got on here to read while I was cooking ground beef for food prep.
 
Shakey said:
Bro, then youll love my Syringe dead space and micro dosing article. i should be posting this weekend. its got a few algebra formulas in it along with dead volume measurements pulled from multiple studies and a technique referenced from a nursing textbook. its sitting at 2100 words, but ill be rewriting and removing a few redundant sections after changing how ill present math and examples.

i aimed for 150mg test weekly, but after measuring my generic pins deadspace with filtered water/food colouring and air locking to deliver dead volume residual waste, im at 234.5mg weekly. airlocking effectively turns a vial yield loss into an overdose by clearing the dead space of compound using a pocket of air during injection. the smaller the volume injected, the larger the vial yield loss.

youll love it. i even added pictures so @HarleyGuy can follow along. 🤣
Click to expand...
Lol, the pictures!
 
HarleyGuy said:
What are anyone's thoughts on adding in some EO with the MCT to further assist solubility? @LevButlerov
EO is technically a solvent but has much broader and safer use in the context of injection subQ and IM and is in a lot of our approved sources higher concentration gear.

@Shakey I think we were talking about this and it was your idea?
Click to expand...
for me I think sterile mct is better than EO but tb really i feel sublingual it should work just fine, even without pins, but I could be wrong
would love some sublingual testing by EVO brothers sisters :D @HarleyGuy
 
Grumpy said:
I could try sublingual as im running low on caps and have raw powder ill give it a go next week see if makes any difference
Click to expand...
see if sublingual makes a difference, open up the caps :D @Grumpy
 
From what I am learning because slu-pp-332 has a logp of 4.5 u would think it would work fine with oil solvents but it has a PSA of around 60-62 which means it wants hydrogen bonding and dipole interactions. Which oil solvents like MCT and EO lack those interactions because they are long hydrocarbon chains. This if mixed with those will only stabilize half the structure and will crash out eventually. So if you look at the characteristics of slu-pp-332 that it's to polar for oil and to hydrophobic for water it needs a mixed solvent system. When u look at the solvent polarity index you have water on one end and MCT and EO on the other end. Slu-pp-332 doesn't like either ends. So u have to look at what's in the middle. You have dmso- ethenol- peg- propylene glycol. This middle ones are your mixed solvents between water and oils. This is where slu-pp-332 falls unfortunately the very trick area. The problem isn't the solvents themselves. They will dissolve and bond but when you dilute them is when they tend to precipitate or crystalize. You don't want to just mix with dmso or peg then inject u need to dilute down these solvents before Injecting. It's even possible if u did inject them just mixed with dmso or peg that they come unbounded and crash inside your body from the water inside us. Apparently that's why molecules that fall into this category tend to be orals.
 
kcates said:
From what I am learning because slu-pp-332 has a logp of 4.5 u would think it would work fine with oil solvents but it has a PSA of around 60-62 which means it wants hydrogen bonding and dipole interactions. Which oil solvents like MCT and EO lack those interactions because they are long hydrocarbon chains. This if mixed with those will only stabilize half the structure and will crash out eventually. So if you look at the characteristics of slu-pp-332 that it's to polar for oil and to hydrophobic for water it needs a mixed solvent system. When u look at the solvent polarity index you have water on one end and MCT and EO on the other end. Slu-pp-332 doesn't like either ends. So u have to look at what's in the middle. You have dmso- ethenol- peg- propylene glycol. This middle ones are your mixed solvents between water and oils. This is where slu-pp-332 falls unfortunately the very trick area. The problem isn't the solvents themselves. They will dissolve and bond but when you dilute them is when they tend to precipitate or crystalize. You don't want to just mix with dmso or peg then inject u need to dilute down these solvents before Injecting. It's even possible if u did inject them just mixed with dmso or peg that they come unbounded and crash inside your body from the water inside us. Apparently that's why molecules that fall into this category tend to be orals.
Click to expand...
It was designed for oral delivery only after all. DMSO though is something i wouldnt inject at all. Its mainly used for flushing bladders. lol And from what ive read DMSO can transport contaminants strongly. Peg looks like the better option with the least contaminant transport? i dont have a background in this stuff, just chase knowledge occassionly so correct me if im wrong.
 
Last edited:
LevButlerov said:
for me I think sterile mct is better than EO but tb really i feel sublingual it should work just fine, even without pins, but I could be wrong
would love some sublingual testing by EVO brothers sisters :D @HarleyGuy
Click to expand...
Being that it's poorly water soluble will enough of it dissolve to cross the mucosa before swallowing the stuff in your mouth. This may be even better but a little out there. Take it rectally. I am not joking about this option. I like to hear the thoughts on that? It would have a partial bypass of the first pass of metabolism and less harsh GI exposure. It would suck to have to stick a pill in the butt a couple of times a week. Kind of makes sense on paper.
 
Shakey said:
It was designed for oral delivery only after all. DMSO though is something i wouldnt inject at all. Its mainly used for flushing bladders. lol And from what ive read DMSO can transport contaminants strongly. Peg looks like the better option with the least contaminant transport? i dont have a background in this stuff, just chase knowledge occassionly so correct me if im wrong.
Click to expand...
Ethanol is also an option actually probably better option on paper. I wonder about peg mix then dilute it with ethanol. Probably have some serious pip to it between both peg and ethanol.
 
kcates said:
Being that it's poorly water soluble will enough of it dissolve to cross the mucosa before swallowing the stuff in your mouth. This may be even better but a little out there. Take it rectally. I am not joking about this option. I like to hear the thoughts on that? It would have a partial bypass of the first pass of metabolism and less harsh GI exposure. It would suck to have to stick a pill in the butt a couple of times a week. Kind of makes sense on paper.
Click to expand...
Hey they have migraine Rx you could technically take as pre-workout. @HarleyGuy asked me to look into it for him.
Screenshot 2026-03-12 223952.webp

so you could slu that way.
kcates said:
Ethanol is also an option actually probably better option on paper. I wonder about peg mix then dilute it with ethanol. Probably have some serious pip to it between both peg and ethanol.
Click to expand...
i bet, this is going to turn into a hybid carrier thread.
 
Demon_throne said:
I run it a little different then most, I normally have to pee about 2 hrs before I wake for the day so I have my first cap then, second upon waking fully for the day 3rd a few hours after and final one about 2 hrs before bed, I keep it away from my weight traning I've found it more effective.

I have a feeling it also depends on what else you are running as well. My set up will remain mostly the same for my upcoming growth phase where by keeping it out of my traning window I avoid it around insulin use

I know @R.AP is also running it during his prep
Click to expand...
The fatigue that I've noticed from Mitochondrial support compounds has come from leveraging the Mots C and/ or SS31 too heavily. I've tested it a few times now, and they're the common denominator. Really yucky. Conservative dosing is the way to go here.

SLU? Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
 
kcates said:
Being that it's poorly water soluble will enough of it dissolve to cross the mucosa before swallowing the stuff in your mouth. This may be even better but a little out there. Take it rectally. I am not joking about this option. I like to hear the thoughts on that? It would have a partial bypass of the first pass of metabolism and less harsh GI exposure. It would suck to have to stick a pill in the butt a couple of times a week. Kind of makes sense on paper.
Click to expand...
I know about rectal use but it's very uncomfortable for most. Lets be honest lol :P guys not going to stick things up their butt @kcates
i tried to have clients test rectal with other products but no one would do it, so sublingual is the best test :D
 
R.AP said:
The fatigue that I've noticed from Mitochondrial support compounds has come from leveraging the Mots C and/ or SS31 too heavily. I've tested it a few times now, and they're the common denominator. Really yucky. Conservative dosing is the way to go here.

SLU? Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
Click to expand...
have you tested sublingual yourself or with clients? I'm have 2 clients test now @R.AP :D
 
R.AP said:
The fatigue that I've noticed from Mitochondrial support compounds has come from leveraging the Mots C and/ or SS31 too heavily. I've tested it a few times now, and they're the common denominator. Really yucky. Conservative dosing is the way to go here.

SLU? Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
Click to expand...
Depending on bioavailability, that could equate to roughly 100-150mg Sub Q. Which would be closing in on the 25mg/kg tested for endurance in mice.
 
R.AP said:
The fatigue that I've noticed from Mitochondrial support compounds has come from leveraging the Mots C and/ or SS31 too heavily. I've tested it a few times now, and they're the common denominator. Really yucky. Conservative dosing is the way to go here.

SLU? Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
Click to expand...
Almost like cooking yourself with too much mots and ss31 in the mix?
 
Shakey said:
i even added pictures so @HarleyGuy can follow along. 🤣
Click to expand...
Algebra formulas and dead space? You mean like if X is dead then Y killed X? Huh?
 
LevButlerov said:
for me I think sterile mct is better than EO but tb really i feel sublingual it should work just fine, even without pins, but I could be wrong
would love some sublingual testing by EVO brothers sisters :D @HarleyGuy
Click to expand...
Sure!! I'll bust open a vial and measure out 1mg (5mg vials) and sublingual that sh*t, no problem!
 
kcates said:
From what I am learning because slu-pp-332 has a logp of 4.5 u would think it would work fine with oil solvents but it has a PSA of around 60-62 which means it wants hydrogen bonding and dipole interactions. Which oil solvents like MCT and EO lack those interactions because they are long hydrocarbon chains. This if mixed with those will only stabilize half the structure and will crash out eventually. So if you look at the characteristics of slu-pp-332 that it's to polar for oil and to hydrophobic for water it needs a mixed solvent system. When u look at the solvent polarity index you have water on one end and MCT and EO on the other end. Slu-pp-332 doesn't like either ends. So u have to look at what's in the middle. You have dmso- ethenol- peg- propylene glycol. This middle ones are your mixed solvents between water and oils. This is where slu-pp-332 falls unfortunately the very trick area. The problem isn't the solvents themselves. They will dissolve and bond but when you dilute them is when they tend to precipitate or crystalize. You don't want to just mix with dmso or peg then inject u need to dilute down these solvents before Injecting. It's even possible if u did inject them just mixed with dmso or peg that they come unbounded and crash inside your body from the water inside us. Apparently that's why molecules that fall into this category tend to be orals.
Click to expand...
Now this is some amazing nerdy shit I haven't heard since University!! I did my M.Sc in Math/Physics but as part of the degree requirements there were prerequisite courses on shit like this and this just brought back memories!

Nice work @kcates!
 
kcates said:
It would suck to have to stick a pill in the butt a couple of times a week.
Click to expand...
Not if it's for EVO research! We don't judge :p
 
kcates said:
Being that it's poorly water soluble will enough of it dissolve to cross the mucosa before swallowing the stuff in your mouth. This may be even better but a little out there. Take it rectally. I am not joking about this option. I like to hear the thoughts on that? It would have a partial bypass of the first pass of metabolism and less harsh GI exposure. It would suck to have to stick a pill in the butt a couple of times a week. Kind of makes sense on paper.
Click to expand...
I’ve seen rectal probiotics that was for more extreme cases
 
R.AP said:
The fatigue that I've noticed from Mitochondrial support compounds has come from leveraging the Mots C and/ or SS31 too heavily. I've tested it a few times now, and they're the common denominator. Really yucky. Conservative dosing is the way to go here.

SLU? Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
Click to expand...
I used it orally no problem, digest no problem, no gi issues too
 
kcates said:
I am up way past my bedtime but my mind is craving and enjoying this problem. I got to try and go to sleep.
Click to expand...
I love this! It's a @Shakey thing to happen to you, I get messages from at 01:00 with deep thoughts on math equations for his dead space article :ROFLMAO:
R.AP said:
Been running 100mg administered orally 4x daily for 20+ weeks now, no issues.
Click to expand...
Awesome thanks @R.AP I've added you to the list for the original post list of members using it.
 
HarleyGuy said:
Now this is some amazing nerdy shit I haven't heard since University!! I did my M.Sc in Math/Physics but as part of the degree requirements there were prerequisite courses on shit like this and this just brought back memories!

Nice work @kcates!
Click to expand...
This all in theory it may not crash out when you mix or inject. Even if it does it will just release slower then anticipated. Some drugs are actually designed to crystalize so that they do slow absorb. I want to try this because it sounds like worse case scenario I will get an irritation. Without proper equipment it's hard to say what really happens. I have heard another guy who is a doctor say that they are making it stable before injection with dmso so it sounds like it can stay stable when it gets diluted. The question is will it stay stable with peg. I guess I won't know until I try it and see if it looks like it stays stable. I guess stay tuned as I learn more and try it. I like the idea of using peg now over dsmo. I wonder if someone already tried peg already and it didn't work and that's why they been using dmso. The benefits of slu-pp-332 out way the negatives of dsmo and that's why they use it.
 
kcates said:
This all in theory it may not crash out when you mix or inject. Even if it does it will just release slower then anticipated. Some drugs are actually designed to crystalize so that they do slow absorb. I want to try this because it sounds like worse case scenario I will get an irritation. Without proper equipment it's hard to say what really happens. I have heard another guy who is a doctor say that they are making it stable before injection with dmso so it sounds like it can stay stable when it gets diluted. The question is will it stay stable with peg. I guess I won't know until I try it and see if it looks like it stays stable. I guess stay tuned as I learn more and try it. I like the idea of using peg now over dsmo. I wonder if someone already tried peg already and it didn't work and that's why they been using dmso. The benefits of slu-pp-332 out way the negatives of dsmo and that's why they use it.
Click to expand...
My understanding is that there isn’t any anecdotal data that I could find of people actually injecting DMSO (maybe a few but nothing of substance) whereas there are some vendors selling SLUPP already suspended in MCT oil with a whole megathread of positive feedback that I’ve read and pointed out to me by @CladHQ.

I think I’m just gonna stick to filtered MCT for now for at least the first vial until we continue to discuss this and figure out the best route.

Can we figure out the polarity of SLUPP and try and find a closely matching polarity carrier that isn’t DMSO?

I like your brain @kcates between you, me, @Shakey and @LevButlerov we might be able to find a better suited carrier than MCT.
 
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